X28C

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Demonstration of use of the time-resolved mixing footprinting apparatus at X28C (modified KinTek stopped flow unit).
First, the two samples to be mixed (shown here: red on the left and blue on the right) are each loaded into "load loops" on opposite sides of the unit. Note that the valve position is set to open for the loading side, but is closed for the opposite side to avoid fluid motion due to pressure from loading of the other side. Once both samples are loaded, the valves are placed in the "drive" position, ready for buffer to push on the loaded sample. A single push moves both samples simultaneously through the T-mixer into the "mixing loop". After the desired mixing time has elapsed, the sample is then pushed through the exposure cell (shown in the back- silver rectangular unit with tubes exiting from both sides) at a rate determined by the desired exposure time and collected (purple sample shown) in a tube below the apparatus.